Hello everyone,
I'm looking for a standard procedure to degradate lignin using laccase in order to reduce the color of lignin.
The method should be as much bio as possible.
As I am not very experienced in the field of bacteria, I'm planning to use commerical laccase (Trametes versicolor, 0.5 U/mg)
In literatur, the methods of the procedure differ very widely, espacially regarding the loading of laccase (U/g of substrate) and the usage of mediators.
Could you suggest any standard procedure or literature?
I appreciate any hint.
Thanks a lot in advance,
David
Dear DAVID BOCHERER
You may find the below article highly helpful to give you complete insight about your problem statement
Article Ligninolytic enzymes and its mechanisms for degradation of l...
Dear David Böcherer thank you for your interesting technical question. As an inorganic chemist I'm not a proven expert in the filed, although we already opublished two papers related to lignin in the past. In addition to the valuable reference suggested by S Kalaiselvan please also have a look at the following potentially useful articles:
Deconstruction of Lignin: From Enzymes to Microorganisms
(see attached pdf file)
and
Role of laccase in lignin degradation by white-rot fungi
https://academic.oup.com/femsle/article/132/3/183/517387
(aslso attached as pdf)
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