We are looking at human-porcine chimeras, and are using anti-human nuclear antigen to stain human cells in these chimeras. Surprisingly, our HuNu stains positive for cytoplasm, and there was no nuclear staining. This antibody is specific for human cells and not for porcine cells. We have used human cells and porcine cells as controls, and both produced desired results with this antibody i.e., positive and negative staining, respectively. I am looking for possible explanations for cytoplasmic staining in our chimeras.
Hi dear, there may be some needed recommendations during processing. Also, using the ANA sometimes is not specific in its action. Read that attachment, it may be helpful.
Thanks
Article International recommendations for the assessment of autoanti...
Dear Dr Raj Aravalli, Could you please point me to your publication with such image or share an image of the human cells in pigs. I would like to test for human cells in pig brains with anti-HuNu antiody. I will appreciate any caveats or appropriate antibody choice. Best Shyam ([email protected])
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