Stability of peptides in bacteria?

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Isam Eldin Hussein Elgailani

Please look at the following below links which may help you in your analysis:

https://aac.asm.org/content/aac/54/9/4003.full.pdf

https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/peptide-stability

Thanks

Rongji Sum

Thanks Isam Eldin Hussein Elgailani but I don't see any mention of the general stability of peptides that are expressed in microbial systems in the references you mentioned.

Pierre-Marie Andrault

I suppose it depends on the strain you want to use for expression. for optimum stability you'll need to choose a protease deficient strain such as BL21 to optimize your peptide stability. You can find other factors that may improve the stability of your recombinant peptide here : https://www.embl.de/pepcore/pepcore_services/protein_expression/ecoli/improving_protein_stability/.

Good luck.