METHOD: What method to use for transient gene silencing in Cucumis sativus ?

I'm planning to silence candidate genes in the root system of C. sativus and viral vectors seem scarce in Cucurbitaceae and with low efficacy. Therefore, I'm looking for people who have experience...

06 December 2020 1,716 1 View

How to define receptor atoms in MOE?

How can a set/define atoms as being part of the receptor for docking in MOE? I have a covalently bonded ligand-receptor complex, and I would like to perform a docking experiment with the complex...

18 October 2020 9,688 2 View

Is someone used a Single or double point method for protein intrinsic viscosity determination?

Dear all, I am wondering if someone uses a single point or double point method to determine the intrinsic viscosity of their proteins. These methods are usually developed for polymers and does...

06 August 2020 6,227 5 View

Dynamic light scattering instruments for several conditions in parallel?

Hello, Our old but not to old DynaPro Plate reader I does not work anymore and Wyatt does not want to investigate the problem as the instrument is 10 years old. We have the money to pay them but...

14 December 2019 6,735 3 View

Why DHB is a reducing agent while sDHB matrix is not a reducing agent?

Hello everyone, My question is the next : Why the DHB matrix is a reducing agent while sDHB matrix is not a reducing agent? In this article it is written that sDHB is a non reducing agent Fast...

19 October 2017 190 3 View

What is the the most validated measure of Sluggish cognitive tempo (SCT)/Concentration deficit disorder (CDD) for 6-12 YO?

What is the the most validated measure of Sluggish cognitive tempo (SCT)/Concentration deficit disorder (CDD) for 6-12 YO? Ideally a questionnaire with a parent and teacher report version

08 February 2017 6,163 1 View

Why do cells round up upon DCFDA (ROS-staining)?

25 November 2014 4,325 6 View

Do you know any good french corpora for named entity detection?

I'm looking for a french corpus tagged with named entities in order to train supervised machine learning. Can you help me to find one?

06 March 2014 7,268 3 View

How can I get the specific RNA expression of an alternative transcript of a gene of interest?

04 November 2013 9,908 6 View

What tools do you use for illustrating your articles?

Each time I write an article, I have to struggle with the problem of the illustrations. I can often see pretty nice figures in articles, more especially in reviews, which makes me think that my...

02 June 2013 3,473 20 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

Help with assigning pressure boundary conditions in FLUENT on a large structure?

Hello, I created a very tall tower (>1000m) and applied pressure boundary conditions at the inlet (bottom) and outlet (top) that correspond to the pressure are their relative altitudes....

22 February 2021 9,972 4 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

What type of pressure sensor are usually used in microfluidics?

My team is designing a dual channel microfluidic chip that runs air and water. I think we've decided on the NE1000 syringe pump for it's versatility and feedback control. We will be ordering the...

18 February 2021 9,928 2 View

Not able to charge-discharge Li-ion coin cell at higher C rate ?

I am working on cathode material. I have made different Li-ion coin cell against Li metal using commercial available cathode materials such as LCO,LMO, LFP and NMC and also with the lab...

18 February 2021 1,450 3 View

How to find the air side pressure drop in a spiral fin tube bundle cross flow heat exchanger?

How to find the air side pressure drop in a spiral fin cross flow heat exchanger? The pressure difference at the inlet and outlet is very low compared to the experimental results, which I...

18 February 2021 1,109 2 View