I have searched for quite a bit, but have not found much. Does anybody know of an antibody for specifically identifying retinal ganglion cells, which works in birds (or one which works in all amniotes/vertebrates)?
I have only found a few papers in which they used an anti-Brn3A antibody. Does anybody have experience with this?
Thank you!
It is hard to find such markers unless someone has published the transcriptome of the RGC in birds.
An alternative approach might be to look for RGC specific genes in rodents and check for sequence similarity in birds.
Some examples are: Thy1 and gamma-synuclein.
http://www.sciencedirect.com/science/article/pii/0306452284901957
http://www.molvis.org/molvis/v14/a184/mv-v14-a184-surgucheva.pdf
You might also want to use the Allen Brain Atlas page to look for more such markers in developing or adult mice and then perform ISH or Immuno in RGCs of birds.
Usually, genes involved in specification would be conserved in expression as well.
Hope this helps.
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