Qiime2- No reads passed the filter. How to set the trunc value?

Hi, I am working on some 16S sequencing data, and seems some of them are low quality. I am not sure how to set the trunc value in dada2. qiime dada2 denoise-paired \ --i-demultiplexed-seqs...

30 January 2021 6,213 3 View

What is the best way to prepare anaerobic medium?

Hi, I would like to prepare anaerobic medium for cultivation of gut microflora. I have an anaerobic chamber. How should I remove oxygen from the medium? Is it enough to leave it for 48 hours in...

05 October 2020 3,093 4 View

Any online tools for metagenomics data analysis, specifically 18S rRNA?

Could you please recommend any online tools for metagenomics data analysis for 18S rRNA?

01 March 2020 5,424 5 View

Is it possible to do SEM on a cross-section cut of a fresh leaf?

Hello, I'm working with pulsed electric field applied to lemongrass leaves to generate pores on the cell membrane to enhance essential oil extraction. I would like to know if there is a way to...

20 October 2019 1,364 3 View

How can I preserve leaf samples to measure the trichome density?

Hi. I'm working in a tropical rainforest and I need to measure the density of trichomes, but due the lack of equipment in the station, I need to preserve the samples during 1 month to then count...

10 October 2019 1,460 4 View

Intracellular staining of peritoneal mast cells for cytokine detection by flow cytometry?

Hi! Can anyone suggest a protocol for intracellular staining of peritoneal mast cells for cytokine detection by flow cytometry?

19 March 2019 9,262 3 View

How long should an autogrid run take?

I've modified the parameters file and managed to launch autogrid. It's been a few hours already, where most tutorial examples take 20 mins.

01 February 2018 950 4 View

Any experience/opinion on sociolinguistic interviews without the interviewer, i.e., only a questionnaire and the interviewees recording themselves?

I want to interview non-native speakers of English, but I am not a native speaker, so to avoid certain implications of this (unnatural setting, accommodation, etc.), I am thinking of sending them...

11 October 2017 4,867 4 View

Does anyone know of a good reference that describes the biology of monocyte/macrophage recruitment and differentiation during wound healing?

Please send me the title/author if you know of a good reference. Thank you!

04 October 2017 7,695 3 View

How do I do immunocytochemistry staining on non-adherent cells?

Are centrifugations required between every wash step/blocking/antibody incubation in order to not lose the cells? Is there a way to make them adhere through a fixative or some other process in...

03 October 2017 8,611 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

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02 March 2021 932 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View