Dear all,
I am trying to find an alternative to southern hybridization for copy number detection in transgenic Arabidopsis. I have attempted the QD PCR protocol (T.Kihara ) for the protocol but I am unable to really understand its rationale.
If anyone can provide me an alternative for copy number detection or provide me a simplistic reasoning behind the gel images from QD PCR, it would be of great help.
Thank you!
Dear Bipasha you can go for Quantitative competitive PCR (QC-PCR) or else Real Time PCR
PNA Pre- Gel Hybridisation
PNA: Peptide Nucleic Acid
PNA binds to DNA at low salt concentration @
Hi Bipasha,
Hope you can find some good information from the below paper:
"Simple Identification of TransgenicArabidopsisPlants Carryinga Single Copy of the Integrated Gene"
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