Southern Hybridisation alternative/ QD PCR analysis for copy number?

Dear all, I am trying to find an alternative to southern hybridization for copy number detection in transgenic Arabidopsis. I have attempted the QD PCR protocol (T.Kihara ) for the protocol but I...

31 December 2019 4,566 3 View

Trancription factor binding site detection software tools?

Can anyone recommend any tool available for tfbs prediction? I am trying to correlated in silico with y2h experiments.

10 November 2019 3,815 2 View

ZDOCK result analysis and interpretation?

I have done protein-protein docking using ZDock. however I am being unable to decipher the output page. Apart from the 5 lines comprising of the header, there are many lines with the docking...

02 March 2019 5,906 1 View

Easy to use softwares for windows for molecular dynamics simulation studies for protein interaction study?Recommendations please?

Could I know any softwares which could be used credibly to go forward with protein protein interaction studies in the form of performing molecular dynamics simulation studies?

06 July 2018 4,166 9 View

Unable to achieve positive transformation of transcription factor?

Hello! So, I have my gene cloned in a PSAT silencing vector which has been validated through sequencing. i have the plasmid in hand and I am trying to transform it into Agrobacterium GV3101 strain...

11 December 2017 2,433 7 View

Too many false positives while cloning in PgemTeasy vector

Hi all, I have been cloning certain transcription factor subunits in Pgem-Teasy vector. However I am facing a huge issue with one factor when I am amplifying it from cDNA. After doing a touchdown...

01 January 1970 9,290 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

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01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What are the important tips for writing review articles?

Important tips for writing a good review articles

24 February 2021 8,544 6 View