Instead of using fotographic films, there is in the market a efficient way to reveal western blotting membranes?
You can take any cooled camera, e.g. BioRad has a nice system if you want to go for HRP conjugated secondary antibodies. And if you don't want to spend that much money, LICOR offers also the C-DiGit Chemiluminescent Western Blot Scanner, a nice small box to get digital images of your Western blots.
And what I like even more is the use of an infrared-scanner (LICOR, Odyssey) and secondary antibodies which are labelled with fluorescence marker coming on 700 nm and 800 nm.
There is also a detection system used by the SpectraMax i3 equipment (Molecular Devices) which is called TRF (time-resolved fluorescence) and requires antibodies conjugated to europium (which are kind of expensive). The main advantage of this strategy would be a reduction in background and unspecific binding.
As said before, Odyssey would be a good option, since you could label different proteins in the same membrane as long as you use antibodies conjugated to different fluorophores.
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