I tried to extract hydrocarbons of water and sediment samples, however, I had couldn't to do the calculation. I did extraction using soxhlet method, then i read the fluorescence of samples using a standard curve but the final value is very high. How I can calculate the real concentration?
Try using the UV absorbance instead. It is easier to quantify using UV absorbance than fluorescence, because there are more interference problems with fluorescence measurements.
Make sure that your samples are clear, not cloudy, and don't have any visible solid material in them. Make sure that your measurements are within the linear range of absorbance of which the spectrophotometer is capable.
Rather than fluorescence why don't you use the EPA's UV method for oils in water?
Use U.V visible spectroscopy(absorption spectroscopy) ( Easy to understand & most reliable for calculating concentrations),
fluorescence deals with transitions from the Excitation states to Ground states(Fluorescence cannot quantify properly)
While absorption(u.v spectra) measures Transition states from the Ground states to Excited states , it is easy to quantify using uv spectroscopy
Maybe all your appreciations are correct, but I have measured by fluorescence and I haven't found any formula that involves all volumes and quantities for soxhlet extraction to sediments and liquid separation to water
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