Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Lymhpoma cell culture issues, help?

Hi! I need help for some issues in growing mantle cell lymphoma cell lines (REC-1, MAVER-1, Granta-519, Mino, Jeko-1). I noticed that it is a little bit hard to maintain a stable cell culture:...

09 February 2021 7,402 11 View

Help to set up an in vitro Bioaccessibility assay?

Hi everyone! i am looking for protocols to evaluate the bioaccessibility of carotenoids in plant matrix. Ideally the best would be a quite easy protocol that could be used massively in the lab,...

02 February 2021 5,516 2 View

Is it possible to get genomic context data on bulk of accessions?

I'm trying to build local gene synteny to identify homologs between different species. To do that, I've been accessing each individual gene of interest, going into gene view in NCBI and...

04 November 2020 9,038 2 View

How seminal was the influence of Giuseppe Campos Venuti and Federico Oliva in Italy, France, Spain and other countries?

The question is what remains in the urban planning and in our professional and intellectual experiences of Giuseppe Campos Venuti and Federico Oliva? What about discipline now? They reasoned and...

30 October 2020 5,450 4 View

Is Sytox (from Thermo Fisher Scientific) a good choice to dectect NETs in flow cytometry using whole blood?

I found several papers in the literature where Sytox (green, red, blue, or orange) is used to detect Neutrophil Extracellular Traps, but it's always used with purified neutrophils. Do you know if...

07 October 2020 5,865 2 View

HPLC Alternative for a new project?

dear collegues, i would like to know if there are any high throughput and specific techniques alternative to the HPLC analysis for protein detection and if possible their relative costs. Thank...

04 October 2020 9,928 3 View

Maximum concentration for HPLC???

Dear Collegues, I'm starting a new project and I need to know what is the maximum concentration for a sample that have to be processed by analytical HPLC. Thank you so much for the answers! Luca

01 October 2020 1,654 5 View

How to make ethyl cellulose water soluble?

Good evening, I'm looking for a way to make ethyl cellulose soluble in water. I read about a low ethoxylated formulation with a DS around 1-1,5 but I cannot find it in any shop. Is there anyone...

20 July 2020 8,592 1 View

How is mathematical giftedness supported in different countries?

I hope for a global overview on mathematical giftedness and its support in school and/or on an extracurricular level. What programmes/opportunities are offered?

08 July 2020 7,124 4 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

01 March 2021 3,905 5 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

What are the harmful effects of these extreme rainfall on the health of newborns? What are the reasons for that?

Extreme rainfall associated with climate change is causing harm to babies in some of the most forgotten places.

01 March 2021 5,647 4 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View