I'll become a student (MSc by research in organic chemistry). I would like to know some useful books expecially for the "work in the laboratory" like spectroscopy, how to purify some reagents & the basic technicals
Thanks a lot.
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Recently I have submitted an article regarding MB dye degradation by ZnO nanoparticle. I received a suggestion to include the discussion on the effect of pH. I want to know what is the impact of...
19 July 2024 4,868 1 View
Hello, I am trying to clone 1334 bp insert into 6.1 kb vector using HiFi DNA assmebly/Gibson assembly kit. I am taking 100ng of vector and 1:5 molar ratio of vector: insert and 4 hrs of...
27 June 2024 9,652 2 View
I am looking for a way to calculate the number of water molecules that are in a hydrogel sample.
16 May 2024 7,064 3 View
I performed two RAD-seq assemblies using ipyrad. I filtered the outputted .vcf file of each of the two assemblies using vcftools to limit the maximum quantity of missing data per locus to 60%. To...
12 March 2024 2,136 0 View
Articles about this topic, statistical methods and analisys for this. What socioeconomic factors are related with higher acces education?. Thanks.
06 December 2023 7,177 6 View
I am having a doubt regarding CMV promoter and SV40 promoter in mammalian expression vector 1. Why CMV and SV40 promoters are used in mammalian expression vectors why not others? 2. There is a CMV...
05 December 2023 1,830 3 View
Me gustaría subir a ResearchGate mi nueva publicación en el formato de pdf Psicoteología: la neurociencia de la fe, para la difusión y divulgación del contenido. Gracias.
05 November 2023 7,352 1 View
El enfoque por competencias en educación, durante los últimos años ha alcanzado gran relieve en los diversos proyectos educativos, sobre todo, porque hay muchos modelos educativos que se han...
11 June 2023 3,550 2 View
Hello, I am trying to achieve the CRISPR KNOCK IN of HDR 1.7 kb in HEK293T will one sgRNA will be efficient to get a knock in or else should I use two sgRNAs?
19 May 2023 9,990 1 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...
09 August 2024 5,444 7 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Hello everyone I am looking for one or some books for propertes and behaviors of stochastic fibre orientation composites. unfotunately I could not find any suitable reference for thias by...
04 August 2024 3,461 3 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...
02 August 2024 7,843 0 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View