Noora Ali Soil Salinity may occur either from poor drainage or from excess amount of soluble salts carried with irrigation water. Several soil physico chemical parameters are also associated (like EC, CEC, Oxidisable OM content etc.) which may result different grades of salinity magnitudes in different types of soil. If your experimental setups are well checked for not having any external contaminations and instruments are rightly calibrated, there should not be any abnormality in depiction of increase &/or decrease in results with the concentration supplied w.r.t. time.
I think it will be beneficial for you if you can once check your instrument again and measure if there is any contamination in your experimental setup and the salinity level of the water you are supplying.
If these are OK, then you may go for soil physico-chemical analysis (pre and post treatment application) for all the 24 pots which might help you relate with any abnormality if persists.
I am adding here some articles and reading materials, hope they might help you in your work.
Thanking You,
Yours faithfully,
Pravat Utpal Acharjee
Water and soil salinity are measured by passing an electric current between the two electrodes of a salinity meter in a sample of soil or water. The electrical conductivity or EC of a soil or water sample is influenced by the concentration and composition of dissolved salts. In order to develop the salinity in pots through salt addition, primarily depends on the soil texture and the level of salinity at which you wish to test the salinity tolerance . You also need to reveal what do you mean by short term , how many days of crop performance you want to evaluate . And which kind of salinity you want to develop artificially for your test plant. The type of salinity will depend upon the type of salinity of that region.
Please take a look at these useful RG links.
Chapter Soil Salinity: Historical Perspectives and a World Overview ...
Chapter Soil Salinity and Salinization Hazard
Chapter Salt-affected Soils: Sources, Genesis and Management
Article Soil salinity development, classification, assessment and ma...
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