Software for genome sequence alignment?

More Ernesto Rios Willars's questions See All

How is the mid-support defined in the running cycle? And how can it be objectively identified?

The mid-stance or mid-support has been defined as much as a phase or event within the race cycle. However, it did not identify a specific definition or a biomechanical indicator that would allow...

10 June 2024 6,257 2 View

Does anyone have a protocol for MPs assessment in fresh snow?

Dear colleagues Could someone send to me an already validated protocol for microplastic analysis in fresh snow that includes sampling and analysis in the lab? I suppose there is no need to digest...

15 May 2024 2,378 1 View

What innovative policies could be used in the rehabilitation and social reintegration of primary prisoners within a prison?

Social reintegration and readaptation goes through processes that begin within prisons through which ex-prisoners achieve reintegration into their community networks and the labor market without...

04 January 2024 4,335 6 View

What is the pH range of a borate-phosphate buffer solution?

The pH range of a borate-phosphate buffer solution can be taken as the range of the phosphate and borate buffers separately?

27 December 2023 8,580 7 View

If an online journal disappeared and its page and repository was deleted, does it no longer count in my curriculum?

A few years ago I published an article on teaching Metrology and the journal is no longer available nor are its archives. If so, should I remove it from my CV?

20 November 2023 5,261 2 View

Buffer TE or PCR grade water for DNA resuspension?

Greetings. In my line of work DNA extraction is common, i had various experiences with both as buffer TE and water, the former is described as the better way to prevent DNA degradation but...

05 November 2023 1,541 3 View

Recomended protocols for metabolite extraction in solid state fermentation?

Greetings. Im searching for protocols for utilization of solid state bioreactors, liquid state are the know standard so information for solid state is more scarce, anyone recomends articles or...

29 October 2023 4,628 0 View

Anyone with experience using utilizing Ammonium Laurate for polyhydroxybutyrate extraction??

Greetings. Im working with PHB extraction and im interested in the use of ammonium laurate as described in this article, but the article itself is scarce on all details, a general protocol would...

11 October 2023 7,697 0 View

What causes the emergence in biology (and other disciplines) from a scientific perspective?

I have a curiosity about the different perspectives on emergence. In biology, we usually assume that there are diverse biological entities, such as cells, tissues, organs, systems, organisms,...

08 September 2023 584 7 View

How can I prevent exosomes from clumping together in PBS for NTA after isolation?

I used the ExoSpin kit to isolate EVs from Plasma using 0.22 double filtration and 10KDa Ultrafiltration device and resuspended in PBS (cacl and mg free) and still find that particles are clumping...

13 August 2023 4,773 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View