Hey!

After the PCR, you have to make a gel to check if the reaction has passed (it's just a yes / no test). Other information you can get is to see how many base pairs you got by how far your sample is from the well after electrophoresis and compare this to a standard (usually there is a template in the ladder instructions). In order to get a sequence in the format .fsa, you need to send for sequencing, for example, I usually send it to Macrogene https://dna.macrogen-europe.com/eng/

I Hope, this is useful for you.

Hi Laurens!

What do you want to do in the end? Perhaps your task can be solved through a gels analysis software?