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Questions related from Nathan Soper
I am trying to optimize my control titration for ITC so that I can ensure my ligand to protein traces are reliable. My small molecule is stored in DMSO. I have been very careful to avoid buffer...
15 February 2024 9,114 3 View
Hello, I have recently switched from doing ligand into protein for ITC because my ligand is in DMSO and has low solubility at 10% DMSO; therefore, now I am doing protein into ligand -- the heat...
10 February 2024 4,530 1 View
Hello, I am having an ITC issue because I cant increase my ligand concentration due to solubility issues. I know there is past literature on reverse titrations being acceptable. I am more asking...
07 February 2024 5,518 2 View
Hello, I am doing ITC and getting repeatable curves that look very promising. After reading some articles, it seems the biggest challenge to avoid is buffer mismatch. Is there a way to rule out...
19 January 2024 1,191 5 View
Hello, I have had moderate success with ITC but most of my issues have been with the Nano Program in fitting. I have a clear curve but the program is not fitting it correctly, does anyone have a...
23 December 2023 9,284 1 View
Hello, I am doing a titration of ligand into protein and am getting good heat releases compared to my ligand into buffer. I am getting more heat release in later injections. Could cooperativity...
08 November 2023 737 4 View
Hello, I am using size exclusion to purify a protein. Before size exclusion a ran my sample through cation exchange. Where I typically see my protein come off during SEC, there was a drop in...
05 April 2022 178 6 View
Hello, I have been using cation exchange (HiTrap SP) for my purifications on ATKA. At that moment we only had two 1mL columns, one was HP and the other XL, and I was using those connected, and my...
01 January 1970 5,298 1 View
