sIPSC and sEPSC in Mice hippocampus?

last month 0 8K Report

Dear Electrophysiologist,

For recording spontaneous inhibitory and excitatory postsynaptic currents (sIPSCs and sEPSCs) in mouse hippocampal neurons, which slicing orientation—coronal or horizontal—better preserves local synaptic circuits?

Badges
Science topic

Similar topics
Social Psychology
Attitude

More Shaimaa Haiba's questions See All

3T3-L1 cells not differentiating ?

Hi, I have been working with 3T3-L1 cells for over a year now.However, over the last couple of months they stopped differentiating into adipocytes. I seed at 200k cells/well (12 well plate), and...

23 May 2024 3,356 1 View

Transfection of MDA MB 231 with miRNA mimic using lipofectamin 2000 ?

What is the optimum concentration to be used for the miRNA mimic and if there is a good working protocol please advice

17 December 2023 5,683 0 View

PCR non-specific binding?

Hi, I have a PCR product for DDL gene for Enterococcus faecalis with non-specific binding as appears in the gel every time I run a gel for the positive control I see two bands the first is my band...

29 October 2023 1,315 3 View

Overload mark on the amplifier multiclamp 700B ?

I use molecular devices setup to patch acute brain slices, and I face this problem of "overloaded"sign that appears on the amplifier, and even when I try to autocomponsate the pipette it shifts to...

09 August 2023 4,032 3 View

Is it possible to perform KEGG pathway analysis with genes without corresponding ENTREZ ID?

Some genes that resulted from my differential gene expression analysis don't have corresponding ENTREZ ID and I want to perform KEGG pathway analysis but most tutorials analyze data with no...

24 July 2023 3,961 2 View

Hello, is it permissible to use only four rats in each group in the experiment design??

What is the minimum number of animals in each group used in expermint?

09 July 2023 5,390 3 View

Can we put a mebrane above the surface of the dura of brain of rats?

I think of applying the drug as a continuous infusion over 36 hours. I thought of putting it on a bio-compatible membrane over the surface of the brain is this possible? has anyone seen a study...

26 April 2023 8,612 0 View

Do anyone have an idea about why my PCR doesnt work ..and how to tackle this issue?

Hi everyone, I have a problem with my PCR and tried so many trials to get my product..I am working on RAG 1 gene in birds...my PCR doesnt work at all..however..I used many recipes and programs to...

27 October 2022 6,814 3 View

I am recently using schrödinger software. I need help to use Espresso with nanopartciles- ligands. Can I find an expert here?

I am following the examples from Schrödinger software but I still face difficulties. It would be nice if I can get help here.

19 May 2022 2,607 0 View

If there any open source (program) to plot the Nanopartciles and attach ligands. From such structure I would like to calculate the DFT?

Dears, If there any open source (program) to plot the Nanopartciles and attach ligands. From such structure I would like to calculate the DFT? kind regards

23 March 2022 3,510 5 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

04 August 2024 4,090 0 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View