How can I simultaneously recognize unknown patterns and variables from a given data set?

20 January 2021 6,385 3 View

What is the difference between "pesticides" and "insecticides"? Are they same?

What is the difference between "pesticides" and "insecticides" in English? Are they the equivalent replacement when used in text? I am an ecological researcher who particularly concerns the...

04 January 2021 6,230 37 View

Viscosity of CsPbI2Br inorganic perovskite solution?

I am trying to fabricate the CsPbI2Br-based inorganic PSC. I used the solution with a concentration of 1.2M following some papers, but I found that the viscosity of the solution is really high....

20 October 2020 6,167 2 View

How to extract membrane protein of platelet ?

I would like to extract membrane proteins of platelets, but I can't find more detailed protocols. How should I extract without a commercial kit?

25 September 2020 1,165 3 View

How can i control the hourglass in lsdyna for ceramic materials?

I am working on ballistic simulation. The first issue is how can i control hourglass for ceramic materials. Because if hourglass exceeded 10% of internal energy, the simulation will be...

11 May 2020 7,494 2 View

Using fixed independent variable across time period?

Hello I'm working on a research on The effect of certain demographic information, Say population density, on people's response at different stage of Covid 19 pandemic. Now the problem is, the...

11 May 2020 7,462 4 View

Is actin or any other housekeeping proteins a suitable loading control in WB in the sequential extraction samples?

Hello guys, I am recently doing WB to detect soluble and insoluble species of a protein using sequential extraction. I have a question about the loading control. Generally, we use...

09 October 2018 6,837 2 View

Why the ZnO I grinded photodegrade slower than bulk?

These days, I do dye degradation experiment. I use mortar and pestle to grind bulk ZnO 10 min, the powders turn yellow. Then use that powder to do dye degradation, why it degrades slower than...

05 August 2018 1,769 4 View

Genomic contamination in RNA extraction with PicoPure RNA extraction kit and Purelink DNase set?

I am working on a project to measure the gene expression on rare cells with a white blood cell background by using the digital PCR technique. The first thing is I need to make sure the tested gene...

21 September 2017 2,012 1 View

How can I idetify the organisms from nitrifying, ammonifying and denitrifying bacteria in lacustrine sedimentary organic matter?

05 November 2016 5,180 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

What statistical test is required for an analysis on pre-COVID & post-COVID patient improvement after attending a 10-week programme?

Hi all, Myself and my supervisor are looking at carrying out a statistical analysis to see whether there is a difference in patient outcomes for our 10-week pain management programme according to...

10 February 2021 6,622 10 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View