Dear all,
I have a question related to embryo rescue. We conducted experiment on wild Cucumis species.When we cultured the half-seed contained embryo, after 3-4 weeks, some seeds germinated but looked abnormal which means we got the plants either formed root or formed shoot . And a white part is always appeared (Please see the attached photos). Then we continued sub-culturing but could not get the normal plants. We think during embryogenesis duration, auxin might be insufficient. We cultured on MS medium without hormone.
Should we try with medium supplemented with auxin?
I am looking forward your comments and advice.
Thank you so much.
You need to cultivate the empryo only without half of the seed, half of the seed is a cotyledon, which is Called , ""Endosperm"" which contains levels of hormones, especially gibberellin. Using only the embryo implant you need to apply hormones ,, but what is the objective of your experience?. yes all the photo until no is upnormal growth, but continuo the expermint to conform that really upnormal, """ compar its growth with full seed""
Dear Mr. Isam Al-madhagi,
Thank you for your answer. Our target is to obtain the plant from culturing the immature embryo. We can not extract only embryo from the seed that is the reason why we try to culture the half-seed. Our problem is that even we got the abnormal germination but after 3-4 times of subculture they will be brown and die.
- Badges
- Science topic