Hey all. I am trying to see if an RDRP protein (about 4 from 1 organism) have NLS. I used the moseslab.csb.utoronto.ca to predict NLS. But I am having some difficulty analyzing the predictions. Each of the proteins have some amino acid sequences, but after I did a multiple sequence alignment, the predicted regions are scattered in different locations. Is the NLS supposed to be conserved or its ok for them to be in different locations on the different proteins (RDRP1,2,3 and 4) in this one organism? thanks