I am using Hi-Media Molecular Biology grade water for my work -- particularly, to reconstitute proteinase K which is in powder form. I have stored it for more than a year I guess, at 4 degree centigrade after I opened it. So, do you think I need to re autoclave it before using it? I think, since I have stored it in its already optimal storing condition, I don't need to re autoclave it.

BTW, it has been filtered using 0.2mm filter. So, I fear that if I will autoclave it, the bottle might contain some contaminant or some DNAse or something and it might contain minute particles and I fear it might interfere in the process.

What is your suggestion?