Hyosub Lee

4 Questions 1 Answers 0 Followers

Questions related from Hyosub Lee

Should I remove the side chains or the entire amino acid to reduce the RSRZ outlier?

Dear all, Hello, I am refining a protein structure obtained from X-ray diffraction. It is ~ 300 aa long but there are about 7-8 consecutive amino acids in the middle with weak electron...

24 March 2025 888 1 View

How is the buffer condition adjusted when conducting Isothermal titration calorimetry (ITC) with Fe siderophore complex?

Hello everyone. In my project, I am dealing with a siderophore binding protein and I need to calculate the dissociation constant of the protein with various siderophores and its precursors with...

05 October 2024 4,605 1 View

Does chelex 100 should be stored in water or other solvents?

I am working with chelex 100 to remove the Fe3+ ions in my media to have it iron free. According to the instruction paper I've got when I bought Chelex 100, it says the chelex 100 should be...

17 March 2022 7,186 0 View

Merging mtz or sca files

Hello, I just diffracted my new crystals and got the structure from Phaser and Autobuild which yields the LLG score of ~3900 and the TFZ score of ~50. When scanning the structure and the...

01 January 1970 998 0 View