How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

How to Adopt / Adapt Research Questions/Instruments Into My Study With Differrent Likert Scale?

Hi All, This question might be a silly question. I wonder how may I adopt/adapt a research questionnaire/instruments into my study? For example, my study is about intellectual capital and SMEs...

03 January 2021 1,443 3 View

How to justify my methodological contribution rather than saying that I will use PLS SEM in my research. May I defend it other ways?

I need to defend my research proposal for the methodological part.

22 September 2020 4,287 3 View

How important to remove negative peak in HPLC for quantitative measurement?

I am using a Bio-Rad column for sugar detection with an RI detector. My mobile phase is 5mM sulfuric acid. When I ran my blank (DI water) I got a negative peak. The baseline was steady before and...

21 July 2020 8,784 1 View

Hi everyone, is there anyone here who did extraction of asccorbyl palmitate from organic solvent?

Im am trying to find the best method for extracting ascorbyl palmitate from organic solvent. Could someone help me?

15 February 2019 3,337 4 View

Why the pH of organic solvent-water mixture is higher than that of aqueous acidic solution?

The pH of organic solvent-water mixture is higher than that of water containing same amount of acid. The pH increases with the increases of organic solvent content in the water- organic mixture...

18 November 2018 630 3 View

Do we need to run normality test (SEM AMOS)?

Hi, I'm in the middle of doing my research report, and am a bit confused with normality test. Since I have very limited knowledge and experience in running SEM AMOS, is that necessary to run...

24 July 2018 245 6 View

Hello, I would like to ask about the explaination for the growth of microbes on membrane filter on agar ?

I've been conducting membrane filtration test for purified water. Instead of colonies forming on the membrane filter , it form continous colonies around the membrane filter. It could be possibly...

26 April 2018 4,801 6 View

What is the expression of Bax and Bcl-2 protein in normal state?

Is it normal when a Bcl-2 protein is highly expressed compared to Bax protein in normal state (control group)? This pattern also appeared in an apoptosis-induced group with higher expression...

17 June 2014 8,279 8 View

No title

I stipped and reprobed my PVDF membrane 4 times using 3 different antibodies (bax, bcl-2 and p53) and loading control (GAPDH), then I stripped and reprobed the same membrane with GAPDH again (as...

14 May 2014 4,940 5 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View