Currently, I am running an HPLC analysis to quantify 4 active compounds in my natural product extract. However, due to a limited budget, I am only able to run 1 concentration of each of the 4 standard compounds. May I know, if I can still quantify the concentration of the 4 active compounds in my extract? As such, I am planning to ratio the peak surface area of the standard compounds with the peak surface area detected from my extract.