What is the maximum number of reads per one run with NGS?

Does anybody know how many reads are usually generated with Illumina NextSeq? For sequencing plasmid library (100-150 bp per read), for example.

27 September 2020 799 2 View

Hello dear researchers, how do you remove the black foil from the vine nursery?

Hello, how do you remove the black foil from the vine nursery? Attached picture in the file. is there machine way or by hand?

04 September 2020 3,566 2 View

Glioblastoma GL-261 cell line not growing s.c?

Hi has anyone had experiance of GL-261 not growing subcutaneously? I have inoculated 10e6 cells in C57BL/6J female mice and the tumors became palpable after a week cca but have subsequently...

19 February 2020 7,543 2 View

Microplastic extraction from fish guts using soluble filters?

Hi there, I want to isolate microplastic particles from fish guts and water samples. My initial idea was to extract guts from selected specimens and then dissolve the whole guts using 10% KOH...

12 February 2020 4,467 8 View

Why are my glucose levels decreasing very rapidly?

Hello, I'm conducting an IPGTT experiment on WT and KO mice (mice which we have shown to have improved glucose tolerance) and I have a serious issue of glucose levels plummeting after 30-60min....

28 September 2018 962 4 View

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10 December 2015 1,475 1 View

How can I calculate deflection of steel fiber reinforced concrete beams?

How can I calculate deflection of SFRC beams (ordinary and high strength)? Is there ACI or other proposals?

11 May 2015 4,977 14 View

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I need approximately 100 meters of ceramic cord (0,5mm). I need it for attaching extensometer to the tensile specimen when conducting tensile test at elevated temperatures.

10 November 2014 4,572 3 View

What is the best bacterial vector for large inserts (around 50 kb)?

Is it possible to insert 50 Kb of DNA to a bacterial vector and successfully maintain and amplify it in bacteria? If so, what would be the best vector?

12 January 2014 8,832 2 View

Where could I find complete interviews of some convicted Western jihadists?

I am NOT looking for studies on the subject, but actual interviews.

01 January 1970 5,977 2 View

Is there any bioinformatic tools or technique for predicting skin cosmeceutical activities of chemical compound?

Except PASS, by using PASS, we can predict biological activities.. Any other tools?

12 January 2021 7,188 1 View

Auricular chondritis with cervical lymphadenopathy of left side in a 20 year old lady can we start anti-TB drugs?

10 December 2020 4,253 3 View

How two compare two exponential data sets?

Hello everyone, I am currently analyzing bacterial growth in cold water dispensers as part of my MSc thesis. During the experiment, I measured the bacterial counts in the effluent water of 3...

15 November 2020 7,628 3 View

How to simulate an automated lubricant dispenser?

Currently I am doing my final year project and I need to simulate an automated lubricant dispenser for a CNC Tank (the tank which located behind the machine). Not to be confuse, I am not modding...

06 November 2020 4,341 3 View

What is a recommended method for simulating paste/slurry (Solid-Liquid) 3D dispensing?

The paste is designed to be dispensed from a syringe with a small nozzle. The paste is composed of highly viscous fluidic phase, and several types of solid spheres with different densities. The...

05 November 2020 2,927 2 View

What is the minimum sample size that is acceptable to conduct a survey?

I'm doing a study to evaluate the adherence rate of clinicians to a clinical practice guideline using clinical vignettes (an online survey). The study only includes physicians who are dealing with...

31 July 2020 5,804 4 View

Assessment of T-cells in the surgically removed Skin tissue ?

How can we assess the presence and amount of T-cells in the freshly removed skin tissue after surgery or punch biopsy? Also, if we freeze the skin tissue at -20C for a long time, after thawing to...

10 July 2020 5,208 4 View

Any body help me to give books, Article, any other forms of materials about indian women in medicine, history of indian physicians for my reference?

Good morning every body, i need books, Article, any other forms of materials about indian women in medicine, history of indian physicians

30 May 2020 9,218 1 View

Does eosin stains collagen in IHCs in typical H&E staining?

Hi all, Does eosin stains collagen in IHCs in typical H&E staining? Please share your thoughts below as comments. Please add a reference if possible (much appreciated). Best, J.

22 May 2020 8,178 2 View

Are there ways to turn some electronics on hand to a ventilator?

Ventilator is running out of supply under COVID-19, especially severely ill patients require non-invasive or even invasive ventilator support. When there is outbreak of COVID-19 locally,...

01 April 2020 5,105 15 View