Can we use distributed acoustic sensing in lab scale (~cm) applications?

I am looking for a way to increase the number of sensors in acoustic emission lab experiments. Fiber optics (DAS) is a natural candidate but the standard resolution is several meters. Is it...

20 September 2020 7,764 4 View

MHCI IP for MS what detachment agent to use?

Dear colleagues, We are planning to run some MHCI IP experiments to identify MHC-bound peptide in adherent cells. I was wondering what do you usually use to detach the cells? I found accutase as...

26 August 2020 2,931 1 View

What is the best way to obtain protein active center for quantum mechanic studies?

Hello there, I am going to model a simple reaction between Fe from heme of human erythrocyte catalase and hydrogen peroxide using QM methods. Before that I need to have valuable structure of...

13 August 2020 8,181 4 View

Which level of theory choose as a reference method?

Hello everyone, I do calculations on compounds with about 25-30 atoms (hydrogen, carbon, oxygen) and about 110 electrons in a water solvent (SMD solvation model). These are optimizations and...

05 June 2020 7,102 10 View

Why does Gaussian converts B3LYP to UB3LYP automatically?

Hello, I'm submit to Gaussian09 input file of molecule as in attached image. The problem is upon execution, when method is automatically changed from B3LYP (restricted by default) to UB3LYP and...

03 December 2019 4,626 2 View

What is the sequence of synthetic miRNeasy Serum/Plasma Spike-In Control cel-mir-39?

26 June 2017 4,629 8 View

How to calculate number of all particles in each cell in Fluent?

Hello, I would like to calculate number of all particles in one cell in during dpm simulation in fluent. Macro P_N(p) gives only number in single stream. Have anyone any idea?

10 May 2016 6,660 9 View

Any advice on a viscosity model for polyesterification phenomena?

To specialized in polyesterification: Does it exist any empirical equation that describes behaviour of viscosity changes as time-dependence function? To be precise, there is a precursor solution...

28 November 2015 7,356 2 View

Is there an organic solvent for TiO2 available?

Is there anybody who can reveal which one of organic solvent (or system) will be suitable to dissolve the titanium dioxide? I'm sure it's possible, but in non-conventional route. Any articles or...

18 December 2014 6,074 4 View

Does anyone have experience with quantity of impurities in XRD pattern?

Is it any reliability in calculating the approximate value of atomic percentage of impurities in sample from XRD studies through dividing the integral part (meant as peaks areas) of dopant by...

12 June 2014 8,720 8 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

How could the concentration from RNA extraction is really low?

Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...

17 February 2021 7,583 4 View