I'd wish to know the shelf life of commercial Taq polymerase under -18C freeze. any help would be greatly appreciated.
I used over six-month expired Taq and it worked well. Those aliquoted nerve thawed after the first freeze. When stored (-20C) properly Taq should be fine for years even after the commercial expires date.
Hey thanks Duminda, my question is what happened if you freez it under -18C. How quickly will it deteriorate - any idea?
Sorry Aiya, I have no idea about that situation.
This question needed to be answered to a civil engineering student studying his first year right now.
22 April 2024 9,118 2 View
Hello all, I am probing for the difference in expression levels of certain proteins involved in fatty acid transport and muscle remodelling in avian skeletal muscle homogenates. I tried using...
03 April 2024 486 0 View
I would like to enhance the torque out of motor by enhancing the dimensions as i have the flexibility to increase the dimensions....In this context I am not changing voltage and current....by...
02 February 2024 319 3 View
It is not mandatory, as it is confident data obtained from my subjects. It might lead to judging the students and the institution they belong to. I have the data, but I doubt whether it is...
16 December 2023 6,187 1 View
I'm an undergraduate and as a part of my research, I need to learn about Natural city and boundary delineation.
12 November 2023 4,499 1 View
Is there any solvent with a high flash point (more than 100 degrees C) that can fully dissolve PLA polymer?
01 November 2023 8,816 0 View
I used linalool standard for estimating total terpenoid content for my plant sample, different linalool concentration has been taken and finally the supernatant has been discarded, the red...
04 June 2023 8,036 2 View
Im a Phd student , currently doing my research on tooth eruption . The statistical analysis required for my work is Probit analysis . Any biostatistician who can provide statistical assistance ?
17 January 2023 5,621 4 View
Nps were synthesized using plant
17 October 2022 8,810 3 View
The performance of SBBR for sewage treatment is investigated in a reactor of capacity 10 L. Conventional K1 kaldnes media (30%) was used as a bio-carrier. after acclimatization for 3 weeks,...
24 October 2021 4,363 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Hi, I'm curious to know if data on chemical compounds from PubChem, such as water solubility properties, can be used to train a machine learning model for commercial purposes. Will this infringe...
30 July 2024 8,707 1 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I need to install this program
25 July 2024 4,756 0 View