I am not an expert in this field, but I am very interested and have researched to find an answer. I received some assistance from tlooto.com for this response. Could you please review the response below to see if it is correct?

To determine sex in mice using PCR, target the Sry gene on the Y chromosome and an autosomal control gene such as Il3. Extract genomic DNA from mouse tissue samples and design primers specific for Sry (e.g., forward: 5'-TGGGACTGGTGACAATTGTC-3'; reverse: 5'-GAGTACAGGTGTGCAGCTCT-3') and Il3 (e.g., forward: 5'-GGGACTCCAAGCTTCAATCA-3'; reverse: 5'-TGGAGGAGGAAGAAGCAGT-3'). Use a standard PCR protocol: initial denaturation at 94°C for 3 minutes, followed by 35 cycles of 94°C for 30 seconds, 60°C for 30 seconds, and 72°C for 30 seconds, with a final extension at 72°C for 5 minutes. This method ensures reliable sex determination by amplifying distinct segments of the X and Y chromosomes, allowing clear differentiation between male and female samples [1][2][3][4].

Reference

[1] McFarlane, L., Truong, V., Palmer, J. S., & Wilhelm, D. (2013). Novel PCR Assay for Determining the Genetic Sex of Mice. Sexual Development, 7, 207 - 211.

[2] Zhang, K., Yang, J., Qin, Z., Lu, T., Lou, D., Ran, Q., Huang, H., Cheng, S., Zellmer, L., Ma, H., & Liao, D. (2022). Establishment of New Genetic Markers and Methods for Sex Determination of Mouse and Human Cells using Polymerase Chain Reactions and Crude DNA Samples. Current Genomics, 23, 275 - 288.

[3] Dreesen, J., Dumoulin, J., Evers, J., Geraedts, J., & Pieters, M. (1995). Multiplex polymerase chain reaction for sex determination of single mouse blastomeres.. Human reproduction, 10 3, 743-8 .

[4] Kima, P., & Rasche, M. (2004). Sex determination using PCR. Biochemistry and Molecular Biology Education, 32.

Thank you, I did not know this platform but now I researched it with elicit and the McFarlain paper was the first hit there too.

Hello there

I suggest you to read this useful article which has detailed procedure about how carry out this.

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