Serum mineral profile of wild carnivore and if leopard this will be very useful?

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Potential Vs current density(mA/cm-2) in EC-Lab?

In Zn-Air Battery, how to plot Potential vs current density(mA/cm-2) in EC-Lab softare/BioLogic instrument. Please suggest. plot of the ref....

04 July 2024 3,807 2 View

What should i use to align molecule for QSAR?

I have molecules with different groups. Some are bis chalcones, some are Dicoumarins, etc. How do I align them in Sybyl software if I can't select any common template?

27 June 2024 7,085 1 View

How to plot Potential vs current density(mA/cm-2) ?

In Zn-Air Battery experiment, how to plot Potential vs current density(mA/cm-2) from discharge voltage curves. Please suggest.

23 June 2024 860 2 View

What method or machine learning algorithm can be used?

I have the molecule's activity value and molecular descriptors. How can I find out which descriptors are the main components for increasing the activity of molecules? also, what Machine learning...

29 May 2024 4,169 2 View

I'm start working in Cryogenic system, particularly using VSM. I am unable to demagnetize the sample. How to do?

When I insert the sample inside VTI, generally, it should show that the magnetic moment is zero(in emu) when the applied magnetic field is zero. But in zero magnetic field displaying magnetic...

25 April 2024 5,234 2 View

Does the Malvaceae family form Rhizobium nodule?

Can any body tell me about Rhizobium nodule reported in the Malvaceae family? So far I know, only rhizobium form nodules in the Fabaceae family.

15 April 2024 6,309 2 View

Can anyone explain me how rainfall intensity data can convert to static rain load while analysing slope stability in ansys software?

In ansys software for slope stability analysis there is no direct input of infilitration rate(as it is in plaxis 2D) if we try to model a slope in ansys 18 how we can quantify rain load ?

03 February 2024 9,432 0 View

Hello Everyone. Can anyone help me with why autodock is not able to find the protein.pdbqt while running autogrid4?

All the files are placed in a specific folder in C drive. (it includes the protein and ligand pdbqt files, autodock4.exe, autogrid.exe and dat files from mgl tools 1.5.7). After repeatedly running...

25 December 2023 5,761 3 View

Whether annealing temperature of standard PCR changes overtime?

Dear Researcher, I facing a lot of problem in PCR amplification of deletion construct of few budding yeast gene, However the The the reaction set up, annealing temp. (55 degree C) standardized...

24 November 2023 8,480 1 View

What could be the problem of tz-mbl assay?

I am doing Tz-mbl assay(beta-galactosidase assay) for evaluating anti-viral activity for hiv infection. When I am doing with p24 assay is coming good but when I am doing it in tz-mbl assay I am...

22 September 2023 5,377 0 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

Can you visualize platelets using EVOS ?

Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...

24 July 2024 9,337 3 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

Detaching human-derived monocytes?

Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...

20 July 2024 7,922 2 View