Anyone knows difference between Primescript and MMLV RT enzymes?

Hi Everybody, Still questions about PCR for circRNA. I do some review for papers and found many of them use MMLV for DNA systhesis. Well in my lab, we usually use Primescript from TAKARA. and I...

04 May 2019 6,808 2 View

Any drugs or chemical can inhibit sox2?

Hi All, I'd like to inhibit sox2 express or down regulated in my current cell culture, but shRNA method seems hard to apply as my cell line is a specifial neuron pregenitor and very hard for...

06 July 2016 6,089 1 View

Colocalization analysis for 3 channels ?

Hi All, I have question about colocalization analysis for 3 channels. I attached an image to make my questions more easily understand. I have 3 images by confocol, from 3 channeal, green, red and...

06 July 2016 9,988 5 View

Subclone PCR, what tag or method can I use to generate long insert piece?

Hi All, My current work needs to insert a large piece DNA into a vector , it's about 5k bp long. We were used invitrogen HF-tag for PCR many years, and it works very good for 1-2k bp insert PCR,...

03 April 2016 6,686 3 View

Did anyone do the electroporation on neurons? or neuron stem cells?

02 March 2016 2,482 4 View

I have RNA integrity gel 2 bands but they are the wrong size.

31 December 2015 8,653 17 View

Why do I get bright GFP-reporter signal but no increase in the mRNA levels?

Hi everyone, I really get a weired problem, I used a plasmid transfection cells, the plasmid has a GFP reporter linked with a protein (lets call it A protein) which I was interested, after...

10 November 2015 3,377 5 View

What if I add another cDNA sequence after the stop code, will it express protein?

Hi All, I was thinking about in translation part, stop code will stop translation, and ATG will start translation, so what if I make a plasmid, it has promoter, then a cDNA start with ATG and add...

03 April 2015 4,878 6 View

Can I put 2 promoters together and hope both of them will function?

Hi All, As my problem, I am working with ES cell lines and want to induce them into neurons. in this process we want to observe it as GFP+ cells. but seems the promoter (i.e ef1a) express high in...

03 April 2015 1,093 3 View

How to lower the pH of a furosemide buffer?

03 April 2015 5,202 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

How to troubleshoot Electrochromic Films and Electrochromic Devices?

I am trying to characterize an electrochromic system. The system uses ITO electrodes on glass, WO3 as the electrochromic film, and NiO as the ion storage layer. When I deposit NiO and WO3 and...

23 February 2021 9,592 2 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Is that possible to do an impedance measurement without FRA module?

One of my colleagues said, "the impedance you can measure with the three electrodes and with the specific sequence varying the frequency" Is it possible to configure the potenciostat without the...

21 February 2021 418 2 View