16 Questions 18 Answers 0 Followers
Questions related from Monica Yiyun Jiang
Hi Everybody, Still questions about PCR for circRNA. I do some review for papers and found many of them use MMLV for DNA systhesis. Well in my lab, we usually use Primescript from TAKARA. and I...
05 May 2019 6,838 2 View
Hi All, I'd like to inhibit sox2 express or down regulated in my current cell culture, but shRNA method seems hard to apply as my cell line is a specifial neuron pregenitor and very hard for...
07 July 2016 6,115 1 View
Hi All, I have question about colocalization analysis for 3 channels. I attached an image to make my questions more easily understand. I have 3 images by confocol, from 3 channeal, green, red and...
07 July 2016 10,022 5 View
Hi All, My current work needs to insert a large piece DNA into a vector , it's about 5k bp long. We were used invitrogen HF-tag for PCR many years, and it works very good for 1-2k bp insert PCR,...
04 April 2016 6,752 3 View
Hi All, As we want to do electroporation in vivo, we design and make our own electrode, first, we use silver, than change to Aluminium. But both of them rusted easily. So I was wonder which...
04 April 2016 8,704 2 View
Hi All, Recently our lab want to delivery plasmids into primary cultured CNS neurons.I have tried lentivirus, lipo transfection before, and now we want to try electroporation. I checked online...
03 March 2016 2,518 4 View
Hi everyone, I extract total RNA for my sample (mouse cell culture) then convert to cDNA and PCR, concentration and A260/280 is 300 ug/ml and 1.97, I feel it quite normal, but no bands even for...
01 January 2016 8,671 17 View
Hi everyone, I really get a weired problem, I used a plasmid transfection cells, the plasmid has a GFP reporter linked with a protein (lets call it A protein) which I was interested, after...
11 November 2015 3,403 5 View
Hi All, I was thinking about in translation part, stop code will stop translation, and ATG will start translation, so what if I make a plasmid, it has promoter, then a cDNA start with ATG and add...
04 April 2015 4,922 6 View
Hi All, As my problem, I am working with ES cell lines and want to induce them into neurons. in this process we want to observe it as GFP+ cells. but seems the promoter (i.e ef1a) express high in...
04 April 2015 1,122 3 View
Hi All, I just prepare 50 mg/ml furosemide in NaOH, then try to adjust pH with HCL. We hope the pH for this buffer is around 7, well we also accept 8 or 9. however, when pH drop a little bit...
04 April 2015 5,229 2 View
Hi All, Now adays I found SYN promoter express high in neurons, so I want to design a plasmid to transfer gene into my mouse ES cell under this promoter (SYN-egfp-puro) then induction them to...
03 March 2015 3,576 1 View
Hi All, I was working with neuronspheres and want to induce them become neuron. IF results shown >95% cell was double label with Sox2 and Nestin, which means there are neuron stem cell. then I...
03 March 2015 1,586 15 View
Hi All, Nowadays I was trying to find a good promoter, then I see the CAG promoter, it seems good, has C( the cytomegalovirus early enhancer element,) as an enhancer, A( chicken beta-actin...
03 March 2015 3,160 6 View
Hi All, Recently I'm working on an AAV plasmid and trying to make new plasmid. And I checked on many website, some said to use stbl3 and some said use stbl2. I got confused about it and didn't see...
12 December 2014 3,482 3 View
Hi everybody, Recently I move on to a new projects about circRNA and a disease, very interesting. My boss got a company to sequencing every published circRNA with patient's samples, around 1000...
01 January 1970 9,384 3 View
