I am relatively new in IHC. My confocal microscope is Zeiss LSM800.
This microscope has a smart setup program with a database to select Fluorophores, and it shows the excitation and emission spectrum of each one and the amount of crosstalk between them.
Is there any application or software to make the same comparison between fluorophores to decide for the best combination for ICH?
Your help is highly appricated.
Masoud
Hi Masoud.
I attach you a link of an spectra viewer that will let you compare among a bunch of fluorochromes. You also will be able to apply filters (excitation/emission wavelenghts, beamsplitters, and filters).
This is the link: https://www.chroma.com/spectra-viewer#tabs-spectra_viewer_plot-left-1
Also, the majority of the fluorochromes manufactoring companies have their own spectra viewers.
Hope this works for you.
با سلام جناب آقای دکتر اکبری
امیدست که حال شما و خانواده محترم خوب و سالم باشد و در کارهای علمی موفق باشید
مدت زمان طولانی است که از شما خبری ندارم. میدانید که ازتباط از طریق فیس بوک میسر نیست. شما می توانید از طریق آدری میل با من تماس داشته باشید.
قربان شما:گروسی
There are thousands of fluorophores to choose from. You will have to provide more info about your application and the wavelength you want to use in IHC.
If you are doing multi-label experiments my suggestion is to use Texas-Red and Alexa 488 combination along with DAPI in the UV channel.
Please provide more info about what you want to do. How many labels, which fluorescent channels etc.
Regards
Thank you, Juan.
Your link is fantastic. It helped me a lot and solved my problem.
Thank you
Masoud
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