Masoud Akbari

5 Questions 12 Answers 0 Followers

Questions related from Masoud Akbari

Why my PCR started to show extra bands and weird results?

Hello guys, I need your help. I used to do PCR for genotyping of my LCK Cre mice. The PCR was working well. You can see in Fig A (Attached picture). No template NT was clean and negative control...

27 November 2020 5,035 3 View

Is there any good protocol for (OTI) CD8 T cell activation?

Dears I need to activate CD8 T cells of OTI mice by OVA peptide. according to this paper (Pulle, G., M. Vidric, and T. H. Watts. 2006. IL-15-dependent induction of 4-1BB promotes...

10 December 2015 8,944 10 View

Which chemokines are involved homing and accumulation of the activated CD8+ T cells in liver?

Hi dears I am transferring in-vitro activated CD8+ T cells into host mice IV. They can enter the liver and accumulate around the infected hepatocytes expressing the antigen. I am wondering which...

26 November 2015 5,270 3 View

Why do my Real time PCR proliferation plots look strange?

Hello everyone I have a problem with real-time PCR. My proliferation plots are strange recently. Please look at this picture: http://postimg.org/image/8e2pa9tg3/ or attached picture. The upper...

20 October 2015 2,303 10 View

How can I generate memory 2C cells by hIL15 in vitro?

Hello. I have a question. I want to generate 2C CD8 memory T cells by culture with hIL15. I need to generate very high number of these cells for cell transfer. I think my protocol is not good. I...

05 January 2015 739 3 View