7 Questions 13 Answers 0 Followers
Questions related from Masoud Akbari
Hello guys, I need your help. I used to do PCR for genotyping of my LCK Cre mice. The PCR was working well. You can see in Fig A (Attached picture). No template NT was clean and negative control...
27 November 2020 5,060 3 View
I am relatively new in IHC. My confocal microscope is Zeiss LSM800. This microscope has a smart setup program with a database to select Fluorophores, and it shows the excitation and...
25 January 2017 3,717 4 View
Hello dears,I am new in the field of FRET imaging. I am doing FRET imaging by Multiphoton microscope system Leica TCS SP5 MP OPO.I use YC3.60 OTI mice. In this mouse CD8+ T cells express CFP and...
24 December 2015 9,090 3 View
Dears I need to activate CD8 T cells of OTI mice by OVA peptide. according to this paper (Pulle, G., M. Vidric, and T. H. Watts. 2006. IL-15-dependent induction of 4-1BB promotes...
10 December 2015 8,950 10 View
Hi dears I am transferring in-vitro activated CD8+ T cells into host mice IV. They can enter the liver and accumulate around the infected hepatocytes expressing the antigen. I am wondering which...
26 November 2015 5,279 3 View
Hello everyone I have a problem with real-time PCR. My proliferation plots are strange recently. Please look at this picture: http://postimg.org/image/8e2pa9tg3/ or attached picture. The upper...
20 October 2015 2,312 10 View
Hello. I have a question. I want to generate 2C CD8 memory T cells by culture with hIL15. I need to generate very high number of these cells for cell transfer. I think my protocol is not good. I...
05 January 2015 746 3 View
