29 August 2024 0 7K Report

I am looking for a comprehensive protocol for immunizing rabbits with a soluble protein. Specifically, I need detailed information on the following aspects:

  • Rabbit Age and Preparation: What is the recommended age for rabbits used in immunization studies, and are there any specific preparation steps required prior to immunization?
  • Immunization Schedule: Could you provide a detailed immunization schedule, including the number of immunization doses, intervals between doses, and any adjuvants that should be used?
  • Spleen Cell/PBMC Isolation: What are the best practices for isolating spleen cells or peripheral blood mononuclear cells (PBMCs) from immunized rabbits for single B cell cloning? Any specific techniques or protocols that ensure high-quality cell isolation would be greatly appreciated.

    • Thank you in advance for your assistance!

    More Karuppiah Valliappan's questions See All
    What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

    I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

    22 July 2024 9,160 2 View

    I am facing problem in the subculturing of hybridoma clone from HAT medium to HT medium?

    I am standardising the methodology for hybridoma production. I did fusion with PEG and cultured in HAT supplemented DMEM+10%FBS. after five days I found several cluster of cells in HAT...

    06 February 2022 4,407 10 View

    How to quantify the diffusion controlled and capacity from CV curve for different scan rate?

    How to distinguished the both the capacitive and diffusion-controlled processes in CV curve.

    30 June 2019 6,046 0 View

    How to estimate the auxin, cytokinin gibberellin and Betain in the liquid seaweed extract ?

    Dear Experts, Kindly suggest me a best protocol to estimate the auxin, cytokinin gibberellin and Betain in the liquid seaweed extract.

    17 April 2018 2,359 5 View

    How to estimate the potassium sulphate in the organic fertilizers ?

    Dear Experts, kindly suggest me a best method to estimate the percentage of potassium sulphate in the organic fertilizers.

    09 April 2018 1,820 12 View

    What is the major difference among charcoal, graphite, graphene and activated carbon?

    Graphite is 3D Graphene is 2D charcoal and activated carbon

    04 April 2018 5,741 4 View

    Why aromatic resin has poor UV resistance?

    Why aromatic resin has poor UV resistance than aliphatic and cycloaliphatic? For aromatic cyclic diacid ,why ortho , meta substitution more durable than para?

    19 January 2018 4,741 2 View

    How can I prepare pure NiO oxide from Ni2O3 black nickle oxide??

    Is there any simple heat treatment steps? or should I follow any chemical methods?

    05 December 2017 2,771 4 View

    Does it possible to prepare the metal sulfides thin films, particularly the substrate temperature fixed at 400 c?

    because the melting Point of the sulphur is low. In this  case If we fixed the temperature of the substrate at 400 c, it may affect the formation of sulphide.

    28 August 2016 5,357 1 View

    Can anyone tell me these species found along the Indian coast ?

    Can anyone tell me these species ? its  found along the Indian coast ?

    23 March 2016 4,900 7 View

    Similar questions and discussions
    What are the long-term impacts of incarceration on youths' developing brain?

    I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

    12 August 2024 862 0 View

    Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

    I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

    09 August 2024 9,621 0 View

    Could it be a cell culture contamination?

    Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

    09 August 2024 2,824 3 View

    Mouse CD3 antibody sequence?

    Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

    08 August 2024 9,157 4 View

    How to confirm the site-directed mutagenesis result without performing NGS?

    I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

    08 August 2024 4,645 2 View

    I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

    After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

    08 August 2024 1,668 3 View

    Is there any way to quantify bacterial and fungal cells in their mixed culture?

    I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

    07 August 2024 7,535 4 View

    Can I use a HisTRAP column for affinity chromatography?

    I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

    07 August 2024 505 3 View

    Do I need specific antibodies to stain for a fusion protein?

    I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

    07 August 2024 5,338 2 View

    How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

    Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

    07 August 2024 8,106 4 View

    Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close