In our lab we were trying to seed the baculovirus onto nanostructures and image them in SEM to get preliminary understanding of virus behavior when they are seeded on top of different nanostructures. For the experiment, we applied an excess of virus solution onto the gold substrate and was incubated at 37 C between 2 to 6 hours, and then rinsed with deionized water, and let the samples dry overnight in the room temperature before observing in the SEM. However, each time the virus start settling and aggregating, and we can’t concentrate the virus because the substrates are fragile and can't handle the centrifuge. Does anybody know any different way to coating the grid?
I have attached some images for the samples after observing in the SEM.
Try using 10% glycerol in the virus solution before drying, it might help stop aggregation
- Badges
- Science topic
- Similar topics
- Chemistry
- Nanotechnology
- Nanostructured Materials