Composition of HEPES buffer solution?

Hi All, I want to make HEPES buffer solution and when I search for the composition I could find two different types of it. Can anyone help me which one is the correct method of HEPES...

08 September 2020 450 4 View

Why my HaCaT cells die after thawed?

I tried to thaw my HaCaT cells several times but 99% of them died. They were frozen with 10% DMSO + 10% FBS + 80% DMEM. I also tried 10% DMSO + 90% FBS then they died less but still only 10-20%...

18 October 2019 1,762 2 View

What could be the factors for unreasonably high heat capacity values from DSC measurements?

I have been running DSC for porous materials but the results I got were quite ridiculous. For the solid porous materials, I obtained heat capacity above that of water, which I cannot reason at...

20 September 2019 2,211 3 View

Do you have training courses to make models for my thesis on climate change and food security in Senegal?

Currently I am a student in Master II at the Faculty of Economics and Management of the University Cheikh Anta Diop Dakar.I finish this higher level with a dissertation whose theme is "the impacts...

01 June 2018 8,422 4 View

How can I prepare an optimal nanoparticles suspension?

13 March 2017 2,927 3 View

POWER: For a longitudinal developmental study with 3 time-points, in order to discriminate between trajectories, what is the minimal sample size?

The study is designed to measure executive function in children in 3 consecutive years. The children will come into the lab annually with their parent. Since executive function is itself a highly...

04 October 2015 7,297 3 View

Does anyone have an answer and references materials on the cause of a short term rise in bottom hole pressure in an injection well?

The bottom hole pressure rise in injection wells.

11 August 2014 5,633 2 View

Could you please help me provide a reference that support the ability to use both instruments in such type of research?

Hi everyone My research examines the effect of using videos on English idiom learning. So, it is regarded a scientific research which means that i should use tests as research instrument....

01 January 1970 1,649 2 View

I really need your help

Could u plz help me in determining variables or criteria that should I depend on in analysing the following questionnaire since I can not analyse and discuss each items separately? Also, all the...

01 January 1970 3,679 4 View

Can i use a slightly different questionnaires in my qualitative research?

From the pilot interview, I have asked some questions that the interviewee is not in their area. He got nervous and shut down his openness to answering the questions.. 1_ Do you think I can ask...

01 January 1970 8,061 9 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View