CyQuant cell prolif. assay - how old can it be and still work??

Hi, I have frozen two 96-well plates with cells that I need to stain using the CyQuant Cell Proliferation Assay from ThermoFisher (C7026). However, I didn't realize that we only have some very old...

06 January 2021 244 4 View

Subgroup analysis in small numbers allowed?

Hello, I had a question regarding subgroup analysis in small patient numbers. I am currently writing a paper on very rare tumours and the response to therapy in a real-world cohort, so no...

18 March 2020 4,983 16 View

Hot to test differences of mean(T test) on all variables in overlapping samples?

Good afternoon, I am working on my thesis on team work and I need to compare if teams differ when I adopt the rule 'at least 2 members of the team gave valid data' (19 teams in total), against...

14 July 2019 5,957 5 View

How do I Obtain Permission to administer the Servant Leadership Questionnaire?

I need to use the SLQ for my dissertation studies.

08 August 2018 1,864 3 View

Anti-FLAG-tag antibody for flow cytometry in mouse without major background signal issues?

Hi there, Can anybody recommend an antibody that works well to detect FLAG-tagged protein in mice with flow cytometry? I am considering to use FLAG tag for generating a transgenic mouse. HIS tag...

26 May 2016 3,404 4 View

Day care quality: Did anyone work with the MELQO - Measuring Early Learning Quality and Outcomes?

We heard that the official version of the MELQO is not yet ready to use. But we wondered whether maybe someone has a research version of it or some other experiences to share. We would like to...

27 April 2016 9,234 1 View

Antibody doesn't work for proteins of HEK lysate?

Hallo, I am doing some western blot experiments at the moment and have some problems. I transfected a construct in HEK293 cells and loaded a lysate to sds gel and blotted the proteins to...

13 April 2016 3,452 9 View

Has anyone used one of the gene trapping constructs form KOMP to generate floxed mice and actually gotten a positive LacZ staining of targeted cells?

Me and two of my colleagues from different labs at my institute used three different constructs targeting 3 different genes. All genes seem to get deleted fairly well but unfortunately no one of...

23 March 2015 8,975 1 View

Why do my infected cells detach in flatbeds and not one by one like they usually do?

Hello. I'm working with rotavirus infections of cells. Usually when I infect with RRV the cells die very fast (as soon as 24 h.p.i) and then they usually detach one by one and you can observe a...

17 March 2015 6,402 2 View

Do you know and use in research any good questionnaire of professional development for preschool/other teachers?

I need a good questionnaire for measuring or valuating (preschool) teacher's professional development - it should be linked with some relevant theories.

16 June 2014 4,419 4 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View