Dear colleagues,
I am searching an appropriate primer for diagnosis of vaginal cancers in female dromedary camels. I encountered some cases with adenocarcinoma, squamous cell carcinoma and some other types of vaginal and cervical cancers. From literature (About half of vaginal cancers in humans is associated with human papillomavirus (HPV) detection, in contrast to cervix cancer which is essentially all associated with HPV). Papillomaviruses have been detected in camels. I tried to test two samples by Prof Robert D Burk (Albert Einstein College of Medicine - NY-USA), but I had a problem in sample transportation. Now, I would like to test the other cases in home, but I do not know what is the appropriate primer that should be used.
Thank you
Ali
Did you try PAP stain and then you can send the stained slides without any problems.
Try FAP59/64 consensus primers or CP4/5 primers. they are often used to amplify also unknown PVs. I would recommend to perform rolling Circle amplification before subjecting DNa to PCr analysis to enrich circular DNa in the Background of liniear host DNA.
https://www.ncbi.nlm.nih.gov/pubmed/10501499