12 December 2019 3 10K Report

I am new to SDS-PAGE and hope someone can help with a persistent problem I am experiencing.

The dye front is stopping about 3/4 of the way down the gel and doesn't seem to move further down than this. Looking at the gel there appears to be a 'ridge' where the dye front is stopping although it doesn't feel like a physical ridge (see photos attached).

I've tried using fresh plates, buffer and protein samples and used a different power pack but this hasn't helped.

Thanks in advance for any advice

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