Screening of oncogene

05 March 2013 1 2K Report

Can anyone tell me whether we can use a soft agar assay or a wound healing assay for the screening of oncogenes?

Hi,

the soft-agar assay can be used for oncogene screening because anchorage-independet growth is one hallmark of transformed cells. I would suggest to do proliferation assay as an alternative to the wound-healing assay because it is not influenced by migration.

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