hi, everybody, I wondered if there is a fastest and sure method or protocol for determining the electroactivity of bacteria and yeast in few day other than potentiostat/galvanostat .
- ACS Synth Biol. 2017 Oct 20;6(10):1860-1869. doi: 10.1021/acssynbio.7b00009. Epub 2017 Jun 21.
Development of a Bacterial Biosensor for Rapid Screening of Yeast p-Coumaric Acid Production.
Transcription factor-based biosensors are used to identify producer strains, a critical bottleneck in cell factory engineering. Here, we address two challenges with this methodology: transplantation of heterologous transcriptional regulators into new hosts to generate functional biosensors and biosensing of the extracellular product concentration that accurately reflects the effective cell factory production capacity. We describe the effects of different translation initiation rates on the dynamic range of a p-coumaric acid biosensor based on the Bacillus subtilis transcriptional repressor PadR by varying its ribosomal binding site. Furthermore, we demonstrate the functionality of this p-coumaric acid biosensor in Escherichia coli and Corynebacterium glutamicum. Finally, we encapsulate yeast p-coumaric acid-producing cells with E. coli-biosensing cells in picoliter droplets and, in a microfluidic device, rapidly sort droplets containing yeast cells producing high amounts of extracellular p-coumaric acid using the fluorescent E. coli biosensor signal. As additional biosensors become available, such approaches will find broad applications for screening of an extracellular product.
- ACS Synth Biol. 2017 Oct 20;6(10):1860-1869. doi: 10.1021/acssynbio.7b00009. Epub 2017 Jun 21.
Development of a Bacterial Biosensor for Rapid Screening of Yeast p-Coumaric Acid Production.
Transcription factor-based biosensors are used to identify producer strains, a critical bottleneck in cell factory engineering. Here, we address two challenges with this methodology: transplantation of heterologous transcriptional regulators into new hosts to generate functional biosensors and biosensing of the extracellular product concentration that accurately reflects the effective cell factory production capacity. We describe the effects of different translation initiation rates on the dynamic range of a p-coumaric acid biosensor based on the Bacillus subtilis transcriptional repressor PadR by varying its ribosomal binding site. Furthermore, we demonstrate the functionality of this p-coumaric acid biosensor in Escherichia coli and Corynebacterium glutamicum. Finally, we encapsulate yeast p-coumaric acid-producing cells with E. coli-biosensing cells in picoliter droplets and, in a microfluidic device, rapidly sort droplets containing yeast cells producing high amounts of extracellular p-coumaric acid using the fluorescent E. coli biosensor signal. As additional biosensors become available, such approaches will find broad applications for screening of an extracellular product.
If you are measuring respiration activity Oxidation Reduction Potential (mv) may be useful to you. I recommend using a Ag/AgCl electrode. The values obtained are referenced in the literature for activity of the biomass. The ORP data obtained are not necesarily directly comparable to other biological systems but are useful in monitoring the system under study.
My question to you is, why are you so bent on short-cuts for such type of analysis. I agree with Anoop and the reference that he has asked you read is excellent. More than that the alternatives that you have mentioned in your question (and wish to bypass) are not only validated but time tested for accuracy.
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