I am working on codon analysis of Prevotella and want to extract pangenome from 28species of Prevotella .Can anyone suggest a scheme by which I would be able to extract the PAN genome of all species together?
So I want to see is the level of a secreted protein in the condition media via western blotting What my doubt is 1. How much cells should I seed and what should be the volume of condition...
19 June 2024 1,927 1 View
this electrode can't be open.
13 May 2024 2,991 3 View
I am trying to express a FAD containing enzyme of mycobacteria in E.coli. I am able to purify the protein which is slightly yellow in color but it seems that my FAD is all unbound to the protein....
16 April 2024 6,912 1 View
I want to prepare reduced form of NADPH. I am using 100mM of Tris (pH8.0) to make the buffer but the absorbance is showing to be 1.6 at 340nm which should supposed to be 0.6. can someone please...
11 March 2024 4,629 3 View
need a. Suggestion for best article related to management studies in data science
07 March 2024 3,506 1 View
Are there specific biochemical tests designed to analyze the ADMET properties of compounds, providing insights into their Absorption, Distribution, Metabolism, Excretion, and Toxicity?
25 December 2023 4,699 2 View
If you want to save time on manual referencing, I have posted 2 hr video on udemy. Mendeley desktop and web version helps us to store, organise and use reference as per any reference style. You...
28 September 2023 5,418 2 View
My reactants are not consumed and no new product spot is formed. My reaction conditions include dissolution of the acetophenone and aldehyde derivative in ethanol followed by dropwise addition of...
24 September 2023 1,908 5 View
To get better digestibility of the plant proteins (Fava bean protein isolates). What heat treatment should I give before the IVD (In vitro digestion) process ?
20 September 2023 7,124 0 View
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17 September 2023 4,135 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View