dear all
my problem is that, when i mix my samle buffer to protein (that i want to migrate), the muxture became coagulated, some forum said that because of SDS, but i dont know how to evoid this problem, does this mean preparing a samle buffer without SDS ??! or there are other solutions??
what i observed that when i heat the coagulated mixture it beacame liquide but i have to load it fastly, even with this methode my sample coagulate in the tips and i can't load only a tiny quantity and subsquently no band or a very weak intensity and deformed band.
I don't know if some one crossed this problem, thank you in advance for your help
greatfully.
leila
Hi Leila,
wat sort of protein u r working with?? is it a bacterial protein?? wats its optimum temperature n pH?
Look 4 them n go through protocol again. I hope u will overcome this.
Good Luck,
Farah.
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