Which heating methods do you recommend for establishing a poultry house (oil heater, gas heater, electric heater)?

I think a gas heater is better for the lower operating costs

03 January 2021 385 18 View

Do you think that the president in Iraq should be able to change the economic situation?

13 December 2020 7,193 3 View

Should I take sample from surface or subsurface for soil analysis?

I am currently doing my final year project on Geochemical analysis of peat samples. I will be looking at parameters such as major and trace elements plus anions and cations. Should i take samples...

27 November 2020 6,668 18 View

What effect do epidemics have on cerebral physiology?

How trauma and fear of infection can affect brain functions and structures?

10 October 2020 6,642 1 View

In your opinion, do I choose a certain disorder to study its relationship with culture, or better not to define the disorder?

Dear colleagues Who can help me find studies or references that study the relationship of culture to mental disorders. I would be very grateful for your help. :)

26 April 2020 3,003 36 View

#Questionnaire, Big data?

Hello, i'm doing about using big data in telecom companies, but i need a questionnaire to measure big data using, can any one give information about that. thanks a lot, Nechirvan Othman Master...

20 February 2020 4,408 3 View

What is best book to start study charge transport in organic semiconductors?

Could you suggest a good book or review articles on electrical transport and mechanisms in organic semiconductors.

17 December 2018 253 2 View

Can oxalate ion oxidaise V(IV) to V(V)?

Greetings I am engaged with the preparation of VO(II) complexes with the different bidentate amine ligands and the oxalate ion. The starting complex (NH4)2VO(C2O4)2 has been prepared by...

30 August 2018 2,028 6 View

Minirhizotron images data set for root?

Hello, I'm working on olive study and I'm using minirhizotron images to estimate root density in soil profile. However, the image analysis software provide an extra data such as, root surface...

19 November 2017 5,621 3 View

Aquapoinic culture system?

Hello, When we use the aquapponic culture system, does the water that come out from the fish tanks has all nutrients (Macro and micro) that the plant need or should we add some? Thanks

11 November 2017 3,224 4 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View