Saffranin and fast green Staining ?

Yahia Othman @Yahia-Othman

19 January 2020 1 4K Report

I need to stain the stem of lily flower (semi-herbaceous). is their any commercial staining that can be used directly.

Also, Is their any updated procedure or commercial product (mixture) of Saffranin and fast green Staining.

Thanks

Brij M Upreti

Article Differential Staining with a Mixture of Safranin and Fast Green FCF

Badges
Science method

More Yahia Othman's questions See All

qui fait des simulations avec SCAPS ?

je voulais développer une recherche sur les nouveaux matériaux des panneaux photovoltaïques en utilisant SCAPS comme simulateur.

27 June 2024 9,999 2 View

Can you provide me a paper about assessment of sysml models in system of systems engineering?

a paper about assessment of sysml models in system of systems engineering containing the following topics: Criteria for evaluating MBSE tools Methodology for comparative evaluation Integration...

17 June 2024 8,267 0 View

Religious translation through cognitive approaches?

How can cognitive approaches help in translating religious texts?

02 June 2024 7,560 1 View

Can i get some political blogs going?

I'm looking for some blogs It is also possible corpus

14 April 2024 2,090 0 View

How to get AIM for whole sample?

Dear respected Professor and experts in this platform. I would like to ask if anyone here has ever encountered the same problem of getting AIM file, specifically on how to get an AIM file of the...

03 April 2024 2,310 0 View

searche a collaboration environment axis ?

Hello i work on water treatment whatever the product i want to improve and develop my axis so i'm looking for a collaboration as soon as possible for a research theme on the environment, e.g....

09 February 2024 8,432 3 View

Electro fenton process ?

how to sunthetize graphene electrodes for the electro Fenton process ?

01 February 2024 7,437 1 View

How i could to determine students’ motivation to learn by student selfassessment?

i know the selfassessment plays a significant role in increasing students’ motivation to learn so i want scale to determine students’ motivation to learn depened on student selfassessment.

22 January 2024 4,413 0 View

Is there a validated questionnaire about caregivers' health-care seeking behaviours for common childhood illnesses ?

Hello everyone, I hope everything is going well with you I'm looking for a validated questionnaire to use in a study to assess caregivers' health-care seeking behaviours for common childhood...

15 September 2023 4,561 0 View

Seeking guidance for validation of a questionnaire?

Dear Academics/expertise I am writing to seek your guidance and validation for the questionnaire that I have developed as a crucial component of my PhD thesis research in management. I kindly...

15 September 2023 8,973 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View