You just need to prepare a PCR reaction using your RNA as a template without doing RT reaction and use a primers that give you a given product from DNA.

Generally , it is difficult to get ride of DNA from RNA sample and itr is necessary you work on primer sequence to give you product from CDNA but it is not possible olways!!

Sa-FA

hi

I think u can have a change with your primers, partial sequence of which is in one extron and the other is in adjacent one.

i agree with Carolin