If my desired band at 225bp ,can i use 100bp DNA ladder ?
Thanks
Dear all, I am using reverse transcription PCR and i have alot of questions: 1-how can i be sure that my RNA samples free from DNA witout DNase treatment (i am using QIAamp blood...
04 May 2010 1,018 3 View
Hello everybody, I would like to ask about testing PCR primer stability and the best way to store lypholized primers.And if the lypholized primers stored at room temperature for two days and...
03 April 2010 6,222 1 View
Hi all I want to make a gene specific primer for my RT-PCR step.I don't know if I need to plug the mRNA sequence from genbank directly into a primer design program to make primer OR if I need to...
03 April 2010 4,720 2 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .
22 February 2021 1,131 4 View
Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...
20 February 2021 6,897 1 View
I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...
15 February 2021 7,444 2 View
I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...
14 February 2021 7,800 7 View
I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...
09 February 2021 8,594 3 View
In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...
08 February 2021 2,792 1 View
Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...
07 February 2021 9,530 3 View
I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...
04 February 2021 1,509 3 View
Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...
04 February 2021 8,993 2 View