Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How to interpret multiple bell shaped curves in Kratky plot?

Hi, I'm trying to analyze a Kratky plot generated from my SAXS data. I am seeing multiple bell-shaped curves in my Kratky plot and I am not able to find any source to get relevant info on this...

20 January 2021 2,474 1 View

Lift off problem with Indium/Gold deposition?

Hi, I'm doing 5/40nm In/Au e-beam evaporation at rates 0.2A/s(for In),1A/s(Au) on lithographically(doubly coated 495k,950k PMMA e-beam resist) done Si substrate at base pressure 2e-6torr. I'm...

18 January 2021 7,550 4 View

How to Import .inp file from abaqus CAE to Ansys perfectly?

I am trying to import an Abaqus .inp file to Ansys but it does not import the whole assembly but only imports a single part. How to solve this.

17 January 2021 5,450 3 View

Can I know the reason for these non specific bands?

a PCR and gel electrophoresis was carried out to obtain the results. they are negative jak2 v617f samples (ref genome - NG_009904 )

13 January 2021 7,658 4 View

What are state-of-the-art methods/techniques/algorithms for the root-cause analysis of the anomalies?

I have been looking for research articles that target the question "why did an anomaly occur?", however, most of the papers that I found are related to "if an anomaly occurred?". I searched Google...

12 January 2021 7,951 3 View

Which third party CFD Codes can be used for FSI Co-Simulation in Abaqus ?

Since Abaqus CFD is no longer available in newer versions what are other available codes for Fluid-Structure Interaction Simulation

11 January 2021 2,012 3 View

Cross country comparison with matched pairs design?

Hi, I'm doing a cross-country comparison. My data for each country has a match pair design i.e. each company within a country is match with a control firm from that country. The data is...

07 January 2021 4,983 6 View

How to determine Viscoelastic and Hyper Elastic Material Properties for Abaqus by mechanical testing ?

I am studying about Impact load resistance property of Polyurea (Elastomer). With respect to this, I need to conduct tests on this material to evaluate its material model behavior as a...

29 December 2020 2,969 1 View

Stably transfected A549 cells are getting washed off during a plate based assay?

HI, I have transfected A549 cells with a plasmid of interest. The transfectants have been stably selected and clonally separated. I am doing a plate based fluorescence assay with the transfectants...

20 December 2020 1,222 4 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Dissertation Topic Ideas for MSc Business Management?

Hello, I hope everyone reading this is doing well during these tough times. I am doing my masters in business management and considering dissertation topics. I'm interested in taking an...

15 February 2021 594 26 View

What could be an interesting topic for a bachelor's thesis in a finance/marketing/computer science field?

Hi everyone! I would like to write my bachelor's thesis on a topic that's currently relevant in the sphere of finance, marketing or computer science (or if it's possible a topic concerning all the...

08 February 2021 1,300 12 View

How to incorporate Monte Carlo simulation in for a real trading data?

Hello, My area of research is to valuate Exchange option (Margrabe option). I am looking for Monte Carlo simulation technique that can be applied to some real trading data. I have evaluated them...

05 February 2021 1,605 4 View

Why has my protein transfer using fresh transfer buffer is worse when compared to protein transfer using old buffer?

Hello, I have performed two westerns in parallel, one using fresh buffer (made with old 10X tris-glycine) and the SDS gel was made 4 days prior to western; and the other western was performed...

03 February 2021 9,311 5 View

Please how can I justify my regression results?

I conducted a study to investigate stock market reaction to equity issues announcement and i ended up with 64 announcements and i then used the announcement day returns as my DV and investigated...

14 January 2021 1,127 3 View

Can you help with the implementation of two different model of deep learning for stock market prediction?

I have stock price data(Open, high, low, close), I want to do prediction by combining two different models like CNN with others. Dataset will be same, what are the ways that I can use for...

04 January 2021 1,845 3 View

Why does my Giemsa staining solution stay in the filter?

I wanted to use commercial Giemsa staining solution and diluted it 1:10 in pH 6.8 Weise buffer. It says to filter the solution, however, most of the stain seems to preticipate in the filter. The...

04 January 2021 5,028 2 View

How can I prepare the erythromycin stock?

I need to prepare a stock of 10 mg / ml erythromycin to check the mic value. I am trying to dissolve 0.1g/10 ml of water but i can't dissolve erythromycin in water. How can i prepare 10 mg/ml...

03 January 2021 9,947 3 View