Does anyone have any experience in culturing the RPMI8226 myeloma cell line? I am using T25 from BD falcon, and what I am observing is a significant fraction of cells are adhering to the plastic and forming colonies rather than growing in suspension.
Mine RPMI8226 are semi adherent too. As longer you grow them you will have more and more adherent cells
I am now culturing this cell line and I had some problems after a week of culture. I also oberved some adherent cells that are growing "healthier" than those ones that I observe in suspension (actually they seem to be burst).
Can you help me with this? Did you kept your flasks vertically or horizontally?
RPMI8226 cells are semi adherent even when you culture them in a non-treated tissue culture flasks, they generally come off easily with a gentle scrape.
I also cultured RPMI8226 cell line but after 1 week it started showing background which reappears again and again. What can be the problem