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Questions related from Neeraj K Satija
We extract protein from tissue or cells, quantify the lysate and then load equal amount of protein on the gel for western blotting. However, it is common at times to observe unequal band...
28 September 2017 6,814 4 View
I tried staining SaOS2 cells with anti-col1 but did not observe any staining under fluorescence microscope. my antibody is working fine since i get staining in hMSCs. Also, I did western to detect...
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I have been trying to detect APC (adenomatous polyposis coli) from RIPA lysates of HEK and SaOS2 cells by western. However, I am not able to detect the protein (Mol Wt ~300kDa). I have tried...
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Does anyone have any experience in culturing the RPMI8226 myeloma cell line? I am using T25 from BD falcon, and what I am observing is a significant fraction of cells are adhering to the plastic...
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I have been working on bone marrow derived mesenchymal stem cells, however, I intend to start work on dental pulp stem cells as well. Firstly, I need advice regarding transportation of dental pulp...
03 February 2013 757 5 View
Hello everyone, I am trying to optimise MOI for transducing hMSCs using TurboGFP lentiviral particles (from Sigma). I incubated cells for 16h at MOI 1,5 & 10, and analysed after 48h by flow...
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