Hi,
I'm evoking CA3 schaffer-collaterals and recording from CA1 pyramidal neurons in slice, at a frequency of 20Hz. I've attached two sample traces: one of them has a baseline that has a rolling baseline that ends up quite far away from the initial baseline, while in the other trace this is less pronounced.
My question is, what causes this rolling baseline? Almost all of the representative traces that I've seen in journals don't have this rolling baseline. Is it because I had a bad clamp in that trace? Aside from monitoring the access resistance and stability of the baseline while recording, are there any other ways to tell if your clamp is poor?
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